Module Details
Module Code: |
ZBIO C3102 |
Module Title:
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Spectroscopy for Biosciences
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Title:
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Spectroscopy for Biosciences
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Module Level:: |
7 |
Module Coordinator: |
Paula Rankin
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Module Author:: |
John Cleary
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Module Description: |
To develop the learners' understanding of the analytical applications of UV-visible (UV-vis) and infrared (IR) spectroscopies and familiarise them with the principles, interpretation, and biologically relevant applications of mass spectrometry (MS), nuclear magnetic resonance (NMR) spectroscopy, and Raman spectroscopy. Learners will also develop their theoretical and practical knowledge in relation to chromatographic methods.
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Learning Outcomes |
On successful completion of this module the learner will be able to: |
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Learning Outcome Description |
LO1 |
Describe the principles, instruments, and applications of various spectroscopic and chromatographic techniques, and interpret the output of these techniques to extract analytically meaningful information. |
LO2 |
Demonstrate practical measurement and analytical skills using a range of laboratory instruments and data acquisition systems. |
LO3 |
Work effectively in a group and demonstrate good communication skills within a group, in presentation format, and in written reporting of laboratory activities. |
Dependencies |
Module Recommendations
This is prior learning (or a practical skill) that is recommended before enrolment in this module.
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No recommendations listed |
Co-requisite Modules
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No Co-requisite modules listed |
Additional Requisite Information
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No Co Requisites listed
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Indicative Content |
Chromatography
System suitability for chromatography - parameters, calculations, and interpretation; van Deemter equation - theory and application
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Spectrophotometric Techniques
Principles, instrumentation, and biologically relevant applications of a range of spectroscopic/spectrometric techniques including ultra violet (UV) infra-red (IR) spectra, mass spectrometry, nuclear magnetic resonance spectroscopy (NMR), Raman spectroscopy.
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Practical
Practical skills will be advanced in chromatographic (TLC/IC/GC/HPLC) separation and analysis, and in spectrophotometric analysis (UV-visible, IR, AAS) and in associated sample preparation procedures.
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Module Content & Assessment
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Assessment Breakdown | % |
Continuous Assessment | 50.00% |
Practical | 50.00% |
AssessmentsFull Time
No End of Module Formal Examination |
Reassessment Requirement |
Exam Board
It is at the discretion of the Examination Board as to what the qualifying criteria are.
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SETU Carlow Campus reserves the right to alter the nature and timings of assessment
Module Workload
Workload: Full Time |
Workload Type |
Workload Category |
Contact Type |
Workload Description |
Frequency |
Average Weekly Learner Workload |
Hours |
Lecture |
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Contact |
Lecture |
12 Weeks per Stage |
2.00 |
24 |
Laboratory |
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Contact |
Practical class |
12 Weeks per Stage |
1.50 |
18 |
Estimated Learner Hours |
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Non Contact |
Independent learning |
15 Weeks per Stage |
5.53 |
83 |
Total Weekly Contact Hours |
3.50 |
Module Resources
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Recommended Book Resources |
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Gordon G. Hammes. (2005), Spectroscopy for the Biological Sciences, Wiley-Interscience, p.192, [ISBN: 9780471713449].
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Douglas Skoog,Donald West,F. Holler,Stanley Crouch. (2013), Fundamentals of Analytical Chemistry, 9th. Cengage Learning, p.1072, [ISBN: 0495558281].
| Supplementary Book Resources |
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Skoog, Douglas A.. (2007), Principles of instrumental analysis, 6th ed..
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Lu-Yun Lian,Gordon Roberts. (2011), Protein NMR Spectroscopy, John Wiley & Sons, p.384, [ISBN: 9781119972013].
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Barbara S Larsen,Charles N. McEwen. (2019), Mass Spectrometry of Biological Materials, CRC Press, p.488, [ISBN: 9780367400583].
| Supplementary Article/Paper Resources |
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Dustin W. Shipp, Faris Sinjab, Ioan
Notingher. (2017), Raman spectroscopy: techniques and
applications in the life sciences, Advances in Optics and Photonics, vol. 9 (2), p.315,
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Purslow Jeffrey A., Khatiwada
Balabhadra, Bayro Marvin J., Venditti
Vincenzo. (2020), NMR Methods for Structural
Characterization of Protein-Protein
Complexes, Frontiers in Molecular Biosciences, vol. 7, article 9, [ISSN: 2296-889X],
| Other Resources |
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Princeton Instruments. A Brief Overview of Raman Spectroscopy
in Life Sciences, Princeton Instruments,
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Yasser M. Moustafa, Rania E. Morsi. (2013), Ion Exchange Chromatography - An
Overview, IntechOpen,
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