Indicative Content |
Buffers in biochemistry
The ionic product of water, buffers and buffering capacity, the Henderson Hasselbalch equation, the preparation of buffers, the blood buffering system
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Enzymology
Classification and mode of action of enzymes. Factors influencing enzyme activity. Introduction to enzyme kinetics; Km, Vmax and Kcat, the Michaelis-Menton and Lineweaver-Burk plots
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Introductory Metabolism
Definition, anabolic and catabolic metabolism. Methods of metabolic control
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Metabolic Pathways
Glycolysis and gluconeogenesis. Introduction to citric acid cycle, the electron transfer chain and β-oxidation. Ketosis. Amino acid catabolism
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Bioenergetics
ATP synthesis. The yield of ATP from central catabolic metabolism
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Practical
The first class will cover Health &Safety regulations. Further classes will include exercises on the following or similar; the preparation and testing of bufffers, basic biochemical extraction and separation, enzymatic assays and the determination of the Km and Vmax of an enzyme. Students will also gain experience in biochemical calculations, plotting curves and laboratory report writing.
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SETU Carlow Campus reserves the right to alter the nature and timings of assessment
Module Resources
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Recommended Book Resources |
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Lubert Stryer,Jeremy Berg,John Tymoczko,Gregory Gatto. (2019), Biochemistry, WH Freeman, p.1208, [ISBN: 9781319114657].
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David Nelson,Michael M. Cox. Principles Biochem 7e (International Ed), [ISBN: 9781319108243].
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Supplementary Book Resources |
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Sareen S. Gropper,Jack L. Smith,Timothy P. Carr. (2016), Advanced Nutrition and Human Metabolism, Cengage Learning, p.640, [ISBN: 9781305627857].
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This module does not have any article/paper resources |
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Other Resources |
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