Module Details

Module Code: ZBIO C3104
Module Title: Molecular Biology and Immunology 2
Title: Molecular Biology and Immunology 2
Module Level:: 7
Credits:: 5
Module Coordinator: Paula Rankin
Module Author:: Ann-Marie Enright
Domains:  
Module Description: The aim of the module is to introduce students to the fundamentals of molecular biology, bioinformatics, medical microbiology and immunology.
 
Learning Outcomes
On successful completion of this module the learner will be able to:
# Learning Outcome Description
LO1 Apply practical skills in the molecular biology and medical microbiology laboratory with respect to CGLP, health and safety, problem solving, team work, efficient record keeping and timely submission of reports
LO2 Discuss basic medical microbiology
LO3 Describe the immune system and the diagnostic applications of immunology.
Dependencies
Module Recommendations

This is prior learning (or a practical skill) that is recommended before enrolment in this module.
No recommendations listed
Co-requisite Modules
No Co-requisite modules listed
Additional Requisite Information
No Co Requisites listed
 
Indicative Content
Practical
Practical classes will develop skills including: The manipulation and handling of recombinant organisms and molecules with emphasis to CGLP and health and safety, monitoring, recording and analysing experimental data in molecular biology, operating the range of instrumentation specified safely and effectively in the laboratory, effective group work and good written and oral communication skills, safety in the molecular biology lab, specific hazards and risks, waste disposal of EtBr, UV visualisation, DNA isolation, DNA quantification and visualisation, gel electrophoresis, restriction enzyme digestion of DNA and cloning, transformation of cells with recombinant DNA molecules, DNA amplification using the polymerase chain reaction, demonstration of the effects of heat and pH on DNA, detection of endotoxins, the LAL test, production of pyrogen-free water, basic serological techniques, agglutination reactions, ELISA testing. The application of bioinformatics to discover variability in sequences and trace the effects of molecular evolution in related genes and proteins.
Medical Microbiology & Immunology:
An introduction to medical microbiology; microorganisms as agents of disease. The human body as a microbial environment. The resident flora and non-specific defence mechanisms. Endooxins and exotoxins. The importance of endotoxins in the pharmaceutical industry. Structure of the Gram Negative cell wall. LAL testing. Monitoring of the factory environment. The immune system, including humoral and cell-mediated immunity. The structure of antibodies. Antibody and antigen reactions. Immediate and delayed hypersensitivity. The use of antibodies as diagnostic agents. Monoclonal antibodies. Agglutination tests. ELISA testing.
Module Content & Assessment
Assessment Breakdown%
Practical40.00%
End of Module Formal Examination60.00%

Assessments

Full Time

No Continuous Assessment
No Project
Practical
Assessment Type Practical/Skills Evaluation % of Total Mark 40
Timing n/a Learning Outcomes 1
Non-marked No
Assessment Description
Performance in Lab class/practicals and practical reports or assignments
End of Module Formal Examination
Assessment Type Formal Exam % of Total Mark 60
Timing End-of-Semester Learning Outcomes 2,3
Non-marked No
Assessment Description
n/a
Reassessment Requirement
Exam Board
It is at the discretion of the Examination Board as to what the qualifying criteria are.
Reassessment Description
Exam Board

SETU Carlow Campus reserves the right to alter the nature and timings of assessment

 

Module Workload

Workload: Full Time
Workload Type Workload Category Contact Type Workload Description Frequency Average Weekly Learner Workload Hours
Lecture Contact Lecture 12 Weeks per Stage 2.00 24
Laboratory Contact Practical class 12 Weeks per Stage 2.00 24
Estimated Learner Hours Non Contact Independent learning 15 Weeks per Stage 5.13 77
Total Weekly Contact Hours 4.00
 
Module Resources
Recommended Book Resources
  • Madigan, MT, Martinko, JM, Bender, KS, Buckley DM, and Brock, T. (2014), Brock’s Biology of Microorgansms, Prentice Hall.
  • Brown TA. (2016), Gene Cloning and DNA Analysis, 7. Wiley Blackwell.
  • Coirco, R, Sunshine, G and Benjamini, E. (2003), Immunology - a short course, Wiley.
  • Brown TA. (2006), Genomics, 3rd Ed. Garland Science.
  • Goldsby, RA, Kindt, TJ, Osborne, BA, and Kuby, J.. (2006), Immunology, 5th. W.H. Freeman.
This module does not have any article/paper resources
This module does not have any other resources
Discussion Note: